【學(xué)術(shù)成果】

 

　　從事微生物功能基因和工業(yè)酶制劑的研發(fā)工作。先后主持參與國家“863”、國家自然科學(xué)基金、省重大專項(xiàng)、省自然科學(xué)基金重點(diǎn)和省發(fā)改委產(chǎn)業(yè)重大等23項(xiàng)課題的研究。獲福建省科技進(jìn)步二等獎2項(xiàng)、福建省科技進(jìn)步三等獎2項(xiàng)、省自然科學(xué)優(yōu)秀論文一等獎1篇、中國科協(xié)優(yōu)秀學(xué)術(shù)論文獎1篇。已在《metabolic Engineering》,《Bioresource Technology》，《Journal of Natural Products》,《Marine Biotechnology》、《Journal of Biotechnology》、《BMC Biotechnology》、《J Agric Food Chem》，《Journal of the American Oil Chemist’s Society》和《微生物學(xué)報(bào)》、《生物工程學(xué)報(bào)》等國內(nèi)外刊物上發(fā)表210多篇論文，授權(quán)與申請專利40多項(xiàng)。重視成果產(chǎn)業(yè)化，建立了多個產(chǎn)學(xué)聯(lián)合體。

 

　　【代表論文】

 

　?。?）Pengchao Wang, xinwei Yang, Baixue Lin*, Jianzhong Huang*, Yong Tao*. Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol.

 

　　metabolic Engineering, 2017, DOI: 10.1016/j.ymben.2017.09.013

 

　?。?）Feng Qi, Mingliang Zhang, Youwei Chen, Xianzhang Jiang, Jinxin Lin, Jianzhong Huang*. A lignocellulosic hydrolyzate-tolerant Aurantiochytrium sp. mutant strain for docosahexaenoic acid production.Bioresource Technology,2017, 227:221-226.

 

　?。?）Feng Qi, Limei Zou, Xianzhang Jiang, Shaoli Cai, Mingliang Zhang, Xinwei Yang, Jian-Zhong Huang*. Integration of heterologous 4-hydroxybenzoic acid transport proteins in Rhodobacter sphaeroides for enhancement of Coenzyme Q10 production.RSC Adwances, 2017,7:17346-17352.

 

　?。?）Yongbiao Zheng, Jiyan Zhang, Liufeng Wei, Mianmian Shi, Jifeng Wang, Jianzhong Huang.  Gunnilactams A-C, Macrocyclic Tetralactams from the Mycelial Culture of the Entomogenous Fungus Paecilomyces gunnii.Journal of Natural Products, 2017, DOI:10.1021/acs.jnatprod.7b00060

 

　?。?）Yongbiao Zheng，Li Xu，Liufeng Wei， Mianmian Shi , Jiyan Zhang，Jianzhong Huang*.A Sphingosine-type Cerebroside in Clavicorona pyxidata induce fruit body formation. Natural Product Research, 2017, DOI: 10.1080/14786419.2017.1308364.

 

　　（6）Wu, Weibin; Guo, Xiaolei; Zhang, Mingliang; huang, Qinggeng; Qi, Feng; Huang, Jianzhong Huang*. Enhancement of L-Phenylalanine Production in Escherichia coli by Heterogeneous expression of Vitreoscilla Hemoglobin, Biotechnology and Applied Biochemistry, 2017, DOI: 10.1002/bab.1605

 

　?。?）Xianzhang Jiang, Hongjiao Liu, Yongchao Niu, ?Feng Qi, Mingliang Zhang, Jianzhong Huang*. Functional Identification and Regulatory Analysis of d6-fatty Acid Desaturase from Oleaginous Fungus Mucor sp. EIM-10.Biotechnology Letters, 2017, 39(3): 453-461.

 

　?。?）Xianzhang Jiang， Hongjiao Liu，Yongchao Niu，Meirong Hu， Feng Qi，Mingliang Zhang，Jianzhong Huang*. Determination of Temperature Sensitive Plasmid Copy Number inEscherichia coli by Absolute and Relative Real-Time Quantitation PCR. Jundishapur Journal ofMicrobiology, 2017，Doi: 10.5812/jjm.14600.

 

　?。?）Qingeng Huang, Ling Liang, Weibin Wu, Songgang Wu, Jianzhong Huang*. metabolic engineering of Corynebacterium glutamicum to enhance L-leucine production. African Journal of Biotechnology, 2017, 16(18):1048-1060.

 

　?。?0）Zhengyu Shu, Hong Lin, Shaolei Shi, Xiangduo Mu, Yanru Liu, Jianzhong Huang*. Cell-bound lipases from Burkholderia sp. ZYB002: gene sequence analysis, expression, enzymatic characterization, and 3D structural model. BMC Biotechnology, 2016,16:38.  DOI: 10.1186/s12896-016-0269-6

 

　?。?1）Zhengyu Shu, Hailong Wu, Hong Lin, Ting Li, Yanru Liu, Fei Ye, Xiangduo Mu, Xin Li, Xianzhang Jiang, Jianzhong Huang*. Decolorization of Remazol Brilliant Blue R using a novel acyltransferase-ISCO (in situ chemical oxidation) coupled system.Biochemical Engineering Journal, 2016, 115:56-63.

 

　　（12）Feng Qi, Lei Sun, Mingliang Zhang, Xianzhang Jiang, Xiao Cao, Jianzhong Huang*. Alpha-linolenic acid production by the mutant Rhodosporidium toruloides. International Journal of Food Engineering, 2016, 2（2）：140-146.

 

　?。?3）Chongrong Ke, Xinwei Yang, Huanxin Rao, Wenchao Zeng, Meirong Hu, Yong Tao, Jianzhong Huang*. Whole-cell conversion of L-Glutamic acid into Gamma-aminobutyric acid by metabolically engineered Escherichia coli. Springerplus, 2016, 5:591-598.

 

　?。?4）Feng Qi, Xuebing Zhao,Yuki Kitahara,Tian Li, Xianjin Ou, Wei Du, DehuaLiu ,Jianzhong Huang. Integrative transcriptomic and proteomic analysis of the mutant lignocellulosic hydrolyzate-tolerant Rhodosporidium toruloides.Eng. Life Sci.2016, DOI: 10.1002/elsc.201500143

 

　　（15）Dongmei Wang, Juan Zeng, Qi Li, Jianzhong Huang, Réjean Couture, Yanguo Hong. Contribution of adrenomedullin to the switchof G protein-coupled μ-opioid receptors fromGi to Gs in the spinal dorsal horn followingchronic morphine exposure in rats. British Journal of Pharmacology, 2016, 173: 1196-1207.

 

　?。?6）Dongmei Wang, Yaping Xue, Yanhua Yan, Minjie Lin, Jiajia Yang, Jianzhong Huang, Yanguo Hong. Reversal of neurochemical alterations in the spinal dorsal horn and dorsalroot ganglia by Mas-related gene (Mrg) receptors in a rat model of spinalnerve injury. Neurobiology of Disease,2016, 91: 274-283.

 

　　（17）Xiao-Jun Ji, Kai-Qiang Mo, Lu-Jing Ren, Gan-Lu Li, Jian-Zhong Huang, He Huang. Genome Sequence of Schizochytrium sp. CCTCC M209059, an Effective Producer of Docosahexaenoic Acid-Rich Lipids.Genome Announcements, 2015, 3(4):e00819-15.  DOI:10.1128/genomeA.00819-15.

 

　?。?8）Yongbiao Zheng, Haiyue Pang, Jifeng Wang，Guowei Shi, Jianzhong Huang. New Apoptosis-inducing Sesquiterpenoids from the Edible Fungus Pleurotus cystidiosus. J Agric Food Chem.,2015，63(2):545-551.

 

　?。?9）Bihong Shi, Hong Zheng, Jianzhong Huang, Xiuzhen Luo, Xiaolei Luo.Purification and partial characterization of a thermostable antimicrobial protein from Bacillus subtilis FB123.World J Microbiol Biotechnol.2015, 31(8):1285-1290. DOI: 10.1007/s11274-015-1871-9.

 

　　（20）Xiaoyuan Hu, Ruoyu Mao, Yong Zhang, Da Teng, Xiumin Wang, Di Xi,Jianzhong Huang*,Jianhua Wang*. Biotechnical paving of recombinant enterocin A as the candidate of anti-Listeria agent. BMC Microbiology, 2014, 14（1）:220-230.

 

　?。?1）Baoyu Tian, Yan Xu, Wanling Cai, Qingeng Huang, Yuanyuan Gao, Xin Li, Jianzhong Huang.Molecular Cloning and Overexpression of an Endo-β-1,4-xylanase Gene from Aspergillus nigerin Industrial Saccharomyces cerevisiaeYS2 Strain.Applied Biochemistry and Biotechnology, 2013, 170(2): 320-328.

 

　?。?2）Li-Na Qin, Fu-Rong Ca, Xin-Rui Dong, Zhen-Bang Huang, Yong Tao,Jian-Zhong Huang*, Zhi-Yang Dong*. Improved Production of Heterogenous Lipase in Trichoderma reesei by RNAimediated Gene Silencing of an EndogenicHighly Expressed Gene. Bioresource Technology, 2012, 109:116-122.

 

　?。?3）Zheng-Yu Shu, Ji-Guang Wu, Lan-Xing Cheng, De Chen, Yong-Mei Jiang, Xin Li, Jian-Zhong Huang*. Production and characteristics of the whole-cell lipase from organic solvent tolerant Burkholderia sp. ZYB002. Appl. Biochem Biotechnol, 2012, 166:536–548.

 

　?。?4）Zhengyu Shu, Jiguang Wu, De Chen, Lanxing Cheng, Yi Zheng, Jianping Chen, Jianzhong Huang*. Optimization of Burkholderia sp. ZYB002 lipaseproduction for pitch control in thermomechanical pulping processes. Holzforschung, 2012，66:341-348.

 

　?。?5）Jian-Zhong Huang*，Xian-Zhang Jiang，Xiao-Feng Xia，Ai-Qun Yu， Ruo-Yu Mao，Xiao-Feng Chen，Bao-Yu Tian. Cloning and functional identification of delta5 fatty acid desaturase gene and its 5′-upstream region from marine fungus Thraustochytrium sp. FJN-10. Marine Biotechnology，2011，13（1）:12-21.

 

　?。?6）Zhengyu Shu，Jiguang Wu，Longyin Xue，Ruifeng Lin，Yongmei Jiang,，Lianghua Tang，Xin Li，Jianzhong Huang*. Construction of Aspergillus niger lipase mutants with oil-water interface independence. Enzyme and Microbial Technology，2011，48（2）：129-133.

 

　?。?7）Yong-Biao Zheng, Dang-XiaChen, Jun-Jing Li, Jiang-Zhong Huang*. New Sesquiterpenoid from Pestalotiopsis sp.Chinese Journal of Natural Medicines, 2011, 9 (6): 421-424

 

　?。?8）Zheng-Yu Shu，Huan Jiang，Rui-Feng Lin，Yong-Mei Jiang，Lin Lin，Jian-Zhong Huang?. Technical methods to improve yield，activity and stability in the development of microbial lipases. Journal of molecular catalysis B-enzymatic，2010，62（1）：1-8.

 

　?。?9）Bao-Yu Tian，Qin-Geng Huang，Yan Xu，Chun-Xiang Wang，Rui-Rui Lv，Jian-Zhong Huang*. Microbial community structure and diversity in a native forest wood-decomposed hollow-stump ecosystem. World J Microbiol Biotechnol，2010，26（2）：233–240.

 

　　（30）Wei Huang，Bao-Yu Tian，Jing Guo，Wan-Ling Cai，Jian-Zhong Huang*.Production of an In Vitro-Derived Deletion Mutation of Brevibacillus laterosporus by Constructing a Homology-Driven Integration Vector. Curr Microbiol，2010，61（5）：401-406.

 

　?。?1）Chenggang Xu, Yong Qin, Yudong Li, Yuetong Ji, Jianzhong Huang, HouhuiSong, Jian Xu. Factors influencing cellulosome activity in Consolidated-Bioprocessing of cellulosicethanol. Bioresource Technology, 2010, 101(24):9560–9569.

 

　　（32）Yi-Ming Tao, Xiang-Zhi Zhu, Jian-Zhong Huang, Su-Juan Ma, Xiao-Bing Wu, Min-Nan Long, Qing-Xi Chen. Purification and properpties of endoglucanase from a sugarcane bagasse hydrolyzing strain Aspergillus glaucus XC9. Agricultural and Food Chemistry, 2010, 58(10):6126-6130.

 

　?。?3）Zhengyu Shu, Ruifeng Lin, Huan Jiang, Yanfeng Zhang,Mingzi Wang, and Jianzhong Huang*. A rapid and efficient method for directed screening of lipase-producing Burkholderia cepacia complex strains with organic solvent tolerance from rhizosphere. Journal of bioscience and bioengineering，2009，107（6）：658-661.

 

　　（34）Bao-Yu Tian，Chong-Rong Ke，Wei Huang，Ke-Qin Zhang，Jian-Zhong Huang*. Direct visualization of bacterial infection process in nematode hosts by an improved immunocytochemical method. World J Microbiol Biotechnol，2009，25：909-912.

 

　?。?5）Baoyu Tian，Wei Huang，Jianzhong Huang，Xianzhang Jiang，Lina Qin. Investigation of protease-mediated cuticle-degradation of nematodes by using an improved immunofluorescence-localization method. Journal of Invertebrate Pathology，2009，101：143–146.

 

　?。?6）Jianzhong Huang*，Xianzhang Jiang，Xiaowei Zhang，Weihua Chen，Baoyu Tian，Zhengyu Shu，Songnian Hu. Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid. Journal of Biotechnology，2008，138：9-16.

 

　?。?7）Jianzhong Huang，Tsunehiro Aki，Toshihiro Yokochi，Toro Nakahara，Daiske Honda，Seiji Kawamoto， Seiko Shigeta，Kazuhisa Ono，Osamu Suzuki. Grouping newly isolated docosahexaenoic acid-producing Thraustochytrids based on their polyunsaturated fatty acid profiles and comparative analysis of 18S rRNA genes.Marine Biotechnology，2003，5（5）：450-457.

 

　?。?8）Jianzhong Huang，Tsunehiro Aki，Kazutaka Hachida，Toshihiro Yokochi，Seiji Kawamoto，Seiko Shigeta，Kazuhisa Ono，Osamu Suzuki. Profile of polyunsaturated fatty acids produced by Thraustochytrium sp. KK17-3. Journal of the American Oil Chemist’s Society，2001，78（6）：605-610.

 

　　（39）Jianzhong Huang，Tsunehiro AKI, Seiji KAWAMOTO, Seiko SHIGETA，Kazuhisa ONO，Osamu SUZUKI. Enzymatic preparation of glycerids rich in docosahexaenoic acid from Thraustochytrid single cell oils by Candida rugosalipase. Journal of Oleo Science，2002，51（7）：447-455.

 

　　【授權(quán)與公開專利】

 

　　1、黃建忠，江賢章，周芬，等；溫度敏感型載體pBBR1MCS-2-Ts3及其應(yīng)用（授權(quán)），專利號： ZL201510340762.0

 

　　2、黃建忠，江賢章，周芬，等；溫度敏感型載體pBBR1MCS-2-Ts1及其應(yīng)用（授權(quán)），專利號：ZL201510340926.X

 

　　3、黃建忠，江賢章，張明亮；利用秸稈水解液發(fā)酵生產(chǎn)二十二碳六烯酸的方法（授權(quán)），專利號：ZL201310571413.0

 

　　4、黃建忠，黃欽耿，吳偉斌，等；一株高產(chǎn)L-纈氨酸工程菌的構(gòu)建及其應(yīng)用（授權(quán)），專利號：ZL201410840584.3

 

　　5、柯崇榕，黃建忠，陶勇，等；產(chǎn)γ-氨基丁酸的重組菌及其構(gòu)建方法與應(yīng)用（授權(quán)），專利號：ZL 201510229161.2

 

　　6、柯崇榕，黃建忠，楊欣偉，等；一株產(chǎn)吡咯喹啉醌生絲微菌及其應(yīng)用（授權(quán)），專利號：ZL 201510151479.3

 

　　7、黃欽耿，黃建忠，梁玲，等；一株高產(chǎn)L-亮氨酸工程菌及其應(yīng)用（授權(quán)），專利號：ZL 201410841171.7

 

　　8、吳偉斌，黃建忠，郭小雷，等；一種L-色氨酸的精制方法（授權(quán)），專利號：ZL201510406813.5

 

　　9、鄭永標(biāo)，黃建忠，林琳，等；一種利用古尼擬青霉生物轉(zhuǎn)化茶咖啡因的方法（授權(quán)），專利號：ZL201310336267.3

 

　　10、鄭永標(biāo)，黃建忠，劉艷如，等；苯炔類化合物及其制備方法及在水稻病原菌防治上的應(yīng)用（授權(quán)），專利號：ZL201210514877.3

 

　　11、鄭永標(biāo)，許小萍，黃建忠，等；一種具有抗菌活性的非蛋白質(zhì)氨基酸及其制備方法（授權(quán)），專利號：ZL201610226061.9

 

　　12、王明茲，陳志紅，黃建忠，等；一種竹材和竹制品專用防霉菌劑的制備及其應(yīng)用（授權(quán)），專利號：ZL201210302047.4

 

　　13、黃祖新，陳由強(qiáng)，黃建忠，等；糖質(zhì)作物清汁發(fā)酵生產(chǎn)乙醇的清潔生產(chǎn)工藝（授權(quán)），專利號：ZL200510076749.5

 

　　14、黃建忠，祁峰，舒正玉，等；無載體固定化米根霉脂肪酶及其制備方法和生產(chǎn)生物柴油的應(yīng)用，  CN104928279A（公開）

 

　　15、黃建忠，祁峰，張明亮，等；一種裂殖壺菌的選育方法及一種裂殖壺菌，CN105331571A（公開）

 

　　16、黃建忠，吳偉斌，郭小雷，等；一株生產(chǎn)L-羥脯氨酸的細(xì)菌及其應(yīng)用，CN105238708A（公開）

 

　　17、黃建忠，祁峰，張明亮，等；一種強(qiáng)化圓紅冬孢酵母產(chǎn)α-亞麻酸的方法，CN105368885A（公開）

 

　　18、黃建忠，柯崇榕，楊欣偉，等；生產(chǎn)吡咯喹啉醌的細(xì)菌及其應(yīng)用，CN106086052A（公開）

 

　　19、黃欽耿，黃建忠，蔣順進(jìn)，等；一種富硒地頂孢霉菌株及其應(yīng)用，CN105969674A（公開）

 

　　20、黃欽耿，黃建忠，吳松剛，等；一種產(chǎn)特異性抗菌肽的酵母工程菌及其發(fā)酵應(yīng)用，CN106754443A（公開）

 

　　21、江賢章，黃建忠，劉洪嬌，等；溫度敏感型載體pBBR1MCS-2-Ts2及其應(yīng)用，CN104894154A（公開）

 

　　22、江賢章，黃建忠，劉洪嬌，等；溫度敏感型載體pBBR1MCS-2-Ts4及其應(yīng)用，CN104962569A（公開）

 

　　23、祁峰， 張明亮，黃建忠，等；生產(chǎn)亞油酸和亞麻酸的圓紅冬孢酵母及其制備方法，CN105950494A（公開）

 

　　24、黃欽耿，黃建忠，吳松剛，等；一種谷氨酸棒桿菌菌株及其在發(fā)酵生產(chǎn)L-色氨酸中的應(yīng)用，CN 106434478 A (公開）

 

　　25、柯崇榕，黃建忠，廖燦；等定向馴化選育高產(chǎn)吡咯喹啉醌的甲基營養(yǎng)菌，CN105624084A（公開）

 

　　26、祁峰，黃建忠，陳學(xué)端，江賢章，張明亮；基因工程化類球紅細(xì)菌及其制備方法和法尼醇的生產(chǎn)方法，CN107119001A（公開）

 

　　27、張明亮，祁峰，黃建忠，等；利用裂殖壺菌生產(chǎn)二十二碳六烯酸的方法，CN105349588A（公開）