近年的科研項(xiàng)目

 

　　1. 國(guó)家自然科學(xué)基金項(xiàng)目（31672244）：刺激隱核蟲(chóng)誘發(fā)宿主產(chǎn)生免疫保護(hù)反應(yīng)的分子篩選，在研，主持。

 

　　2. 國(guó)家重點(diǎn)研發(fā)計(jì)劃政府間國(guó)際科技創(chuàng)新合作重點(diǎn)專(zhuān)項(xiàng)（2016YFE0121900）：PD-L1  KRAS 信號(hào)通路相關(guān)性及其臨床應(yīng)用，在研，參與。

 

　　3. 國(guó)家自然科學(xué)基金項(xiàng)目（31040084）：刺激隱核蟲(chóng)的表面抗原和胸苷激酶的鑒定和特征分析，已結(jié)題，主持。

 

　　4. 福建省自然科學(xué)基金重點(diǎn)項(xiàng)目：海洋魚(yú)類(lèi)寄生纖毛蟲(chóng)的流行病學(xué)調(diào)查與分子免疫學(xué)分析，已結(jié)題，主持。

 

　　5. 福建省自然科學(xué)基金項(xiàng)目：長(zhǎng)角血蜱谷氨酰胺轉(zhuǎn)胺酶的生物功能及應(yīng)用價(jià)值探討，主持。

 

　　6. 福建省科技廳JK項(xiàng)目：重組刺激隱核蟲(chóng)14-3-3蛋白在刺激隱核蟲(chóng)免疫診斷和預(yù)防中的應(yīng)用價(jià)值探討，已結(jié)題，主持。

 

　　7. 福建省自然科學(xué)基金項(xiàng)目：微小牛蜱半乳糖凝集素的鑒定和特征分析，已結(jié)題，參與。

 

　　8. 福建省重大專(zhuān)項(xiàng)子專(zhuān)題：福建省養(yǎng)殖大黃魚(yú)刺激隱核蟲(chóng)病綜合防控技術(shù)研究與示范，參與。

　　已發(fā)表的主要論文（* 為通訊作者）

 

　　1.Lin Q, M.Yang, Z. Huang, W. Ni, G. Fu, Z. Wang, X. Huang*. Cloning, expression and molecular characterization of a 14-3-3 gene from a parasitic ciliate, Cryptocaryon irritans. Veterinary parasitology. 197(3-4):427-35. 2013.

 

　　2.Huang X*, Y. Xu, G. Guo, Q. Lin, Z. Ye, L. Yuan, Z. Sun, W. Ni. Molecular characterisation of an actin depolymerising factor from Cryptocaryon irritans. Parasitology. 140 (5): 561-568. 2013.

 

　　3.Huang X*, Z. Sun, G. Guo, C. Zheng, Y. Xu, L. Yuan, C. Liu. Cloning and molecular characterization of a surface antigen from Cryptocaryon irritans. Experimental parasitology. 130 (3): 189-194. 2012.

 

　　4.Zhang, G., X. Huang, D. Boldbaatar, B. Battur, B. Battsetseg, H. Zhang, L. Yu, Y. Li, Y. Luo, S. Cao, Y. Goo, J. Yamagishi, J. Zhou, S. Zhang, H. Suzuki, I. Igarashi, T. Mikami, Y. Nishikawa, X. Xuan. Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. Vaccine. 28: 7243–7247. 2010.

 

　　5.M.K. Islam, N. Tsuji, T. Miyoshi, M.A. Alim, X. Huang, T. Hatta, K. Fujisaki. The Kunitz-like modulatory protein haenangin is vital for hard tick blood feeding success. Plos Pathog. 5 (7). e1000497, 2009.

 

　　6.Huang X., N. Tsuji, T. Miyoshi, S. Nakamura-Tsuruta, J. Hirabayashi, and K. Fujisaki.. Molecular characterization and oligosaccharide binding properties of a galectin from the argasid tick Ornithodoros moubata. Glycobiology. 17(3): 313-323, 2007.

 

　　7.Huang X., N. Tsuji, T. Miyoshi, M. Motobu, M.K. Islam, M.A. Alim, and K. Fujisaki. Characterization of glutamine: fructose-6-phosphate aminotransferase from the ixodid tick, Haemaphysalis longicornis, and its critical role for host blood feeding. International Journal of Parasitology. 37(3-4): 383-392, 2007.

 

　　8.Abdul Alim M, N. Tsuji, T. Miyoshi, M. K. Islam, X. Huang, M. Motobu, K. Fujisaki. Characterization of asparaginyl endopeptidase, legumain induced by blood feeding in the ixodid tick Haemaphysalis longicornis.Insect Biochem Mol Biol. 37(9):911-22, 2007.

 

　　9.Motobu M., N. Tsuji, T. Miyoshi, X. Huang, M. K. Islam, M. A. Alim, K. Fujisaki. Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis.FEBS J. 274(13):3299-312, 2007.

 

　　10.Huang X., X. Xuan, R.A. Verdida, S. Zhang, N. Yokoyama, L. Xu, and I. Igarashi. Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses. Clinical Vaccine Immunology. 13(5): 553-555, 2006.

 

　　11.Huang X., X. Xuan, N. Yokoyama, Y. Katayama, T. Anzai, and I. Igarashi. evaluation of enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infections. Veterinary Parasitology. 140(1-2): 158-161, 2006.

 

　　12.Huang, X., X. Xuan, L. Xu, S. Zhang, N. Yokoyama, N. Suzuki, and I. Igarashi. Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses. Journal of Clinical Microbiology. 42: 359-361, 2004.

 

　　13.Huang, X., X. Xuan, H. Hirata, N. Yokoyama, L. Xu, N. Suzuki,and I. Igarashi. Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats. Journal of Clinical Microbiology. 42: 351-353, 2004.

 

　　14.Kumar, S, N. Yokoyama, J. Kim, X. Huang, N. Inoue, X. Xuan, I. Igarashi, and C. Sugimoto. expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton. Molecular and Biochemical Parasitology. 133: 221-227, 2004.

 

　　15.Huang, X., X. Xuan, N. Yokoyama, L. Xu, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, and I. Igarashi. High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential in immunodiagnosis. Journal of Clinical Microbiology. 41: 1147-1151, 2003.

 

　　16. Huang X, X. Xuan, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, T. Mikami, and I. Igarashi. Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clinical Diagnostic Laboratory Immunology. 9: 1343-1347, 2002.