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姓名：王啟要	性別：
民族：	年齡：
最高學(xué)歷：	學(xué)位：
生物化學(xué)與分子生物學(xué)專業(yè)：碩士生導(dǎo)師　　微生物學(xué)專業(yè)：碩士生導(dǎo)師　　生物工程與技術(shù)專業(yè)：碩士生導(dǎo)師　　生物工程領(lǐng)域：工程碩士生導(dǎo)師　　華東理工大學(xué)生物工程學(xué)院，生物反應(yīng)器工程國家重點(diǎn)實(shí)驗(yàn)室副教授，碩士生導(dǎo)師。2012年入選國家鲆鰈類現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系疾病防控崗位后備人才，2013年入選上海市科技啟明星人才計(jì)劃。2013.12-2014.12，美國哈佛醫(yī)學(xué)院/霍華德·休斯醫(yī)學(xué)研究院Matthew K. Waldor教授實(shí)驗(yàn)室訪問學(xué)者。　　主要研究方向：　　1. 采用系統(tǒng)生物學(xué)方法研究動(dòng)物病原性細(xì)菌致病機(jī)制　　主要針對(duì)病原性細(xì)菌的典型流行菌株，采用生化分離純化、轉(zhuǎn)座子隨機(jī)突變體文庫、突變體確定文庫、組學(xué)（基因組學(xué)、蛋白質(zhì)組學(xué)和RNA組學(xué)）以及二代測(cè)序技術(shù)等策略鑒定其毒力因子，然后采用信號(hào)傳導(dǎo)研究方法研究其受胞內(nèi)外以及宿主各種信號(hào)/脅迫下的各種調(diào)控機(jī)制，描繪病原菌在致病過程以及環(huán)境適應(yīng)過程中不同的毒力調(diào)控網(wǎng)絡(luò)，從而為防治藥物和新型疫苗的設(shè)計(jì)和組裝提供靶標(biāo)和相關(guān)分子器件。目前主要研究興趣是遲鈍愛德華氏菌和溶藻弧菌中三型分泌系統(tǒng)（TTSS）和六型分泌系統(tǒng)（T6SS）及其調(diào)控機(jī)制研究。目前這些研究工作與國外大學(xué)（如美國哈佛醫(yī)學(xué)院、賓夕法尼亞大學(xué)、威斯康辛大學(xué)、瑞典Ume? University、英國諾丁漢大學(xué)等）相關(guān)課題組聯(lián)系較為緊密。　　2. 疫苗應(yīng)用開發(fā) 　　主要針對(duì)海洋養(yǎng)殖動(dòng)物病原遲鈍愛德華氏菌和其他病原菌進(jìn)行疫苗的商業(yè)化開發(fā)工作，并且進(jìn)行多聯(lián)疫苗的應(yīng)用研究。這些研究與國內(nèi)水產(chǎn)養(yǎng)殖相關(guān)院校、研究所和企業(yè)緊密合作。　　學(xué)術(shù)兼職：　　1. 澳大利亞微生物學(xué)會(huì)（ASM）會(huì)員，2011-現(xiàn)在　　2. Infect Immun, Vet Microbiol, Microbiology, International Journal of Food Microbiology, J Appl Microbiol, Fish Shellfish Immun, PLOS One, African Journal of Microbiology Research (AJMR), Chinese Journal of Oceanology and Limnology, Diseases of Aquatic Organisms, Antonie van Leeuwenhoek Journal of Microbiology等雜志審稿人

　　承擔(dān)和參加的主要科研項(xiàng)目：

　　1. 國家自然科學(xué)基金項(xiàng)目“VI型分泌系統(tǒng)（T6SS）在溶藻弧菌中調(diào)控堿性絲氨酸蛋白酶合成/分泌的分子機(jī)制”，30901113，2010-2012 (主持)

　　2. 國家自然科學(xué)基金項(xiàng)目“溶藻弧菌中RseA-RpoE 介導(dǎo)PpkA/PppA 調(diào)控群體感應(yīng)和毒力的分子機(jī)制”，31372560，2014-2017(主持)

　　3. 上海市科委科技啟明星人才計(jì)劃項(xiàng)目“遲鈍愛德華氏菌中基于全基因組/轉(zhuǎn)錄組信息的T3SS和T6SS效應(yīng)蛋白的系統(tǒng)鑒定和作用機(jī)制研究”，13QA1401000, 2013.07-2015.06 (主持)

　　4. 農(nóng)業(yè)部行業(yè)公益性專項(xiàng)“東海漁業(yè)資源評(píng)價(jià)與增殖養(yǎng)護(hù)技術(shù)研究”子課題“流行病學(xué)調(diào)查和增殖放流健康標(biāo)準(zhǔn)”，nyhyzx-201303047，2013.01-2017.12 (主持)

　　5. 農(nóng)業(yè)部國家鲆鰈類產(chǎn)業(yè)技術(shù)體系“疾病防控崗位”，CARS-50, 2012-2016 (參加)

　　6. 國家863計(jì)劃主題項(xiàng)目“遲鈍愛德華氏菌活疫苗的產(chǎn)業(yè)化開發(fā)及應(yīng)用”， 2013AA093101，2013.1-2017.12 (參加)

　　發(fā)表論文：

　　2009年9月1日以后的成果(通訊作者*或第一作者)

　　1. Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, and Yuanxing Zhang* Characterization of Edwardsiella tarda rpoS: effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression, Applied Microbiology and Biotechnology, 2009, 83:151-160.

　　2. Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu, Yuanxing Zhang. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS ONE, 2009, 17, 4(10): e7646.

　　3. Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang*. Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish & Shellfish Immunology, 2009, 27(3): 469-477.

　　4. Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, and Yuanxing Zhang*. QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology. 2011, 30: 944-953

　　5. Huan Liu,?Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi,?Yuanxing Zhang*. Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine, Applied Microbiology and Biotechnology, 2011, 91:353-364

　　6. Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, and Yuanxing Zhang*. Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda . Letters in Applied Microbiology. 2011, 53(4):430-437

　　7. Yamin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, and Yuanxing Zhang. Genetic Relationships of Edwardsiella Strains Isolated in China Aquaculture Revealed by rep-PCR Genomic Fingerprinting and Investigation of Edwardsiella Virulence Genes. Journal of Applied Microbiology, 2011, 111(6): 1337-1348 (Q2)

　　8. Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang*. Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology. 2012, 194:439-452 (Q3)

　　9. Lili Sheng, Qiyao Wang*, Qin Liu, Yuanxing Zhang Connection between Type VI Secretion System, Quorum Sensing, and Alternative Sigma Factor in Fish Pathogen Vibrio alginolyticus. Archives of Microbiology, 2012, 194(5):379-390

　　10. Yuanzhi Lv, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang, Qiyao Wang*. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Veterinary Microbiology. 2012, 157:190-199

　　11. Jingfan Xiao, Tao Chen, Qiyao Wang*, Yuanxing Zhang. Comparative analysis of the roles of catalases KatB and KatG in the physiological fitness and pathogenesis of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology. 2012, 54, 425-432 (Q3)

　　12. Minjun Yang, Jingfan Xiao, Haizhen Wu, Yuanzhi Lv, Huajun Zheng, Qin Liu, Yuanxing Zhang, Qiyao Wang*. Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLOS ONE. 2012, 7(5): e36987.

　　13. Jingfan Xiao, Xin Wang, Tao Chen, Yuanxing Zhang, Qiyao Wang*. Identification of qseEGF genetic locus and its roles in regulating hemolytic activity and invasion in fish pathogen Edwardsiella tarda. Letters in Applied Microbiology, 2012, 55(2):91-98

　　14. Huan Liu, Dan Gu, Lili Sheng, Qiyao Wang*, Yuanxing Zhang. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Letters in Applied Microbiology, 2012, 55:73-81

　　15. Yuanzhi Lv, Junyuan Zheng, Minjun Yang, Qiyao Wang*, Yuanxing Zhang. An Edwardsiella tarda mutant lacking UDP-glucose dehydrogenase shows pleiotropic phenotypes, attenuated virulence, and potential as a vaccine candidate. Veterinary Microbiology, 2012, 160: 506-512

　　16. Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA, Veterinary Microbiology, 2013, 162: 652-662

　　17. Yamin Wang, Weizheng Yang, Qiyao Wang*, Yuanxing Zhang. Functional characterization of Edwardsiella tarda twin-arginine translocation system and its potential use as a biological containment for live attenuated vaccine in marine fish vaccinology. Applied Microbiology and Biotechnology, 2013, 97(8): 3543-3557

　　18. Yamin Wang, Qiyao Wang*, Minjun Yang, Yuanxing Zhang. Proteomic analysis of a twin-arginine translocation deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda. FEMS Microbiology Letters, 2013, 343:145-155.

　　19. Yuanzhi Lv, Kaiyu Yin, Shuai Shao, Qiyao Wang*, Yuanxing Zhang. Comparative proteomic analysis reveals new components of the PhoP regulon and highlights a role for PhoP in the regulation of genes encoding the F1F0 ATP synthase in Edwardsiella tarda, Microbiology-SGM, 2013, 159: 1340-1351. (Q2)

　　20. Jingfan Xiao, Tao Chen, Bin Liu, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Edwardsiella tarda mutant disrupted in type III secretion system and chorismic acid synthesis and cured of a plasmid as a live attenuated vaccine in turbot. Fish & Shellfish Immunology, 2013, 35: 632-641. (Q1)

　　21. Minjun Yang, Shuai Shao, Jingfan Xiao, Qiyao Wang*, Yuanxing Zhang. Phylogenetic investigation of Edwardsiella tarda with multilocus sequence typing (MLST) and pulsed field gel electrophoresis typing (PFGE) methods. Aquaculture, 2013, 410-411:79-85. (Q1)

　　22. Yamin Wang, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Presenting a foreign antigen on live attenuated Edwardsiella tarda using twin-arginine translocation signal peptide as a multivalent vaccine. Journal of Biotechnology, 2013, 168:710-717(Q2)

　　23. Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu, Qiyao Wang*, Yuanxing Zhang. An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Appl Micbriol Biotechnol, 2015, 99:1765–1777 (Q1)

　　24. Shuai Shao, Qiliang Lai, Qin Liu Haizhen Wu, Jingfan Xiao, Zongze Shao, Qiyao Wang*, Yuanxing Zhang. Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: propose a novel species as Edwardsiella anguillarum sp. nov. Systematic and Applied Microbiology, 2015, 38: 36-47. (Q2)

　　出版專著：

　　王啟要, 劉琴, 張?jiān)d. 群體感應(yīng)——細(xì)菌之間的語言. 生物科學(xué)與工程 (葉勤主編), 北京, 科學(xué)出版社, 2008, 43-67.

　　專利：

　　1 劉琴, 張?jiān)d, 楊朝, 王啟要. 細(xì)菌表面展示新系統(tǒng)、方法及應(yīng)用. ZL 101182516, 2008.05.21

　　2 劉琴, 張?jiān)d, 楊朝, 王啟要. 新型細(xì)菌表面展示系統(tǒng)、方法及應(yīng)用. ZL 101195823, 2008.06.11

　　3 張?jiān)d, 王鑫, 王啟要, 劉琴. 一種遲鈍愛德華氏菌野生毒株的無標(biāo)記基因缺失減毒突變株及其應(yīng)用, ZL 200910052707.6

　　4 王啟要, 張?jiān)d, 劉歡, 劉琴. 一種溶藻弧菌減毒株的無標(biāo)記基因缺失減毒突變株、相關(guān)制劑其應(yīng)用, 中國發(fā)明專利. 申請(qǐng)?zhí)枺篫L 201110266453.5

　　5 王啟要, 陳濤, 王鑫, 肖婧凡, 劉琴, 張?jiān)d. 遲鈍愛德華氏菌野生毒株的無標(biāo)記基因缺失減毒株、相關(guān)制劑及其應(yīng)用, ZL 201010541646.2

　　6 劉琴，張?jiān)d，周凌云，王啟要. 一種多效價(jià)活疫苗、其制備方法及應(yīng)用. 中國發(fā)明專利. 申請(qǐng)?zhí)枺?00910054113.9

　　7張?jiān)d, 王啟要, 劉琴, 吳海珍, 肖婧凡. 遲鈍愛德華氏菌天然弱毒株及其應(yīng)用. 中國發(fā)明專利. 申請(qǐng)?zhí)枺?01210240313.5

　　8 王啟要, 劉琴, 張?jiān)d, 王亞敏, 吳海珍, 肖婧凡. 遲鈍愛德華氏菌突變株及其應(yīng)用, PCT國際專利: PCT/CN2012/076961

　　9 王啟要, 王亞敏, 劉琴, 吳海珍, 肖婧凡, 張?jiān)d. 魚用多效價(jià)活疫苗及其應(yīng)用, 中國發(fā)明專利. 申請(qǐng)?zhí)枺?01210492313.4

　　10 劉琴, 張?jiān)d, 鄭雁, 王啟要. 一種抗鰻弧菌和遲鈍愛德華氏菌的多效價(jià)活疫苗、相關(guān)表達(dá)載體及應(yīng)用. 中國發(fā)明專利. 專利號(hào)：201010600524.6

　　11 肖婧凡, 張?jiān)d, 劉和松, 王啟要, 劉琴. 遲鈍愛德華氏菌快速檢測(cè)試紙條及快速檢測(cè)方法與應(yīng)用. 中國發(fā)明專利. 申請(qǐng)?zhí)枺?01010568826.X

　　12 吳海珍, 張萌, 李小勇, 沈斌兵, 王啟要, 張?jiān)d. 遲鈍愛德華氏菌免疫保護(hù)性抗原、相關(guān)表達(dá)載體、疫苗和應(yīng)用. 中國發(fā)明專利. 專利號(hào)：ZL201110095826.7

　　本科和研究生教學(xué)：

　　1. 生物科學(xué)專業(yè)大三第2學(xué)期課程，生物信息學(xué)，32學(xué)時(shí)，2學(xué)分，專業(yè)必修課；

　　2. 博士研究生專業(yè)課程：微生物細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)，32學(xué)時(shí)，2學(xué)分，專業(yè)選修課；

　　3. 2008級(jí)生物技術(shù)081班班導(dǎo)師；2012級(jí)生物技術(shù)121班班導(dǎo)師。

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王啟要