張?jiān)d

個(gè)人簡(jiǎn)介
科研項(xiàng)目
學(xué)術(shù)論文
獲獎(jiǎng)
專利
承擔(dān)教學(xué)
研究生培養(yǎng)

姓名：張?jiān)d	性別：
民族：	年齡：
最高學(xué)歷：	學(xué)位：
張?jiān)d 　　生物工程與技術(shù)專業(yè)：博士生導(dǎo)師/碩士生導(dǎo)師　　生物化工專業(yè)：博士生導(dǎo)師/碩士生導(dǎo)師　　發(fā)酵工程專業(yè)：碩士生導(dǎo)師　　生物化學(xué)與分子生物學(xué)專業(yè)：博士生導(dǎo)師　　生物工程領(lǐng)域：工程碩士生導(dǎo)師　　1982年1月畢業(yè)于華東化工學(xué)院化學(xué)工程系，獲學(xué)士學(xué)位。1984年7月畢業(yè)于華東化工學(xué)院生物化工系，獲碩士學(xué)位，1995年9月評(píng)為華東理工大學(xué)教授。1997年擔(dān)任博士生導(dǎo)師。1998年享受國(guó)務(wù)院政府特殊津貼。　　現(xiàn)任職務(wù)有生物工程學(xué)院院長(zhǎng)，上海海洋動(dòng)物疫苗工程技術(shù)研究中心主任，上海阿華生物工程研究所所長(zhǎng)，任國(guó)務(wù)院學(xué)位委員會(huì)生物工程臨時(shí)學(xué)科評(píng)議組專家，曾任國(guó)家863高技術(shù)計(jì)劃海洋技術(shù)領(lǐng)域?qū)＜医M副組長(zhǎng)。受聘為生物反應(yīng)器工程國(guó)家重點(diǎn)實(shí)驗(yàn)室學(xué)術(shù)委員會(huì)副主任，國(guó)家海洋食品工程技術(shù)研究中心專家指導(dǎo)委員會(huì)副主任，福建省海洋生物資源開發(fā)利用協(xié)同創(chuàng)新中心學(xué)術(shù)委員會(huì)副主任，教育部水產(chǎn)品安全重點(diǎn)實(shí)驗(yàn)室、中科院海洋生物資源可持續(xù)利用重點(diǎn)實(shí)驗(yàn)室、農(nóng)業(yè)部海洋漁業(yè)資源可持續(xù)發(fā)展重點(diǎn)實(shí)驗(yàn)室、農(nóng)業(yè)部農(nóng)業(yè)微生物資源利用綜合性重點(diǎn)實(shí)驗(yàn)室、農(nóng)業(yè)部海洋與河口漁業(yè)資源及生態(tài)重點(diǎn)開放實(shí)驗(yàn)室、農(nóng)業(yè)部東海海水健康養(yǎng)殖重點(diǎn)實(shí)驗(yàn)室、農(nóng)業(yè)部東海與遠(yuǎn)洋漁業(yè)資源開發(fā)利用重點(diǎn)實(shí)驗(yàn)室學(xué)術(shù)委員會(huì)委員。擔(dān)任Bioresouces and Bioprocessing、Journal of Biochemistry and Molecular Biology Research、中國(guó)生物制品學(xué)雜志、中國(guó)抗生素雜志、中國(guó)海洋藥物、食品安全與質(zhì)量檢測(cè)學(xué)報(bào)編委。目前承擔(dān)國(guó)家自然科學(xué)基金重點(diǎn)項(xiàng)目和面上項(xiàng)目，擔(dān)任國(guó)家現(xiàn)代農(nóng)業(yè)技術(shù)體系(鲆鰈類)崗位科學(xué)家。為本科生講授上海市精品課程生物化學(xué)，為博士研究生開設(shè)生物反應(yīng)器設(shè)計(jì)與放大課程，已指導(dǎo)59名碩士研究生和45名博士研究生畢業(yè)，6名博士后出站，正在指導(dǎo)碩士研究生12名，博士研究生7名，博士后2名。已在國(guó)內(nèi)外發(fā)表論文380余篇，其中SCI收錄175篇，出版著作2部，參編著作6部，獲省部級(jí)科技進(jìn)步獎(jiǎng)6項(xiàng)，中國(guó)石油和化學(xué)工業(yè)優(yōu)秀科技圖書一等獎(jiǎng)1項(xiàng)，申請(qǐng)國(guó)家發(fā)明專利52項(xiàng)，其中獲得授權(quán)43項(xiàng)；申請(qǐng)國(guó)際專利3項(xiàng)，其中獲得授權(quán)美國(guó)專利1項(xiàng)。　　學(xué)術(shù)梯隊(duì)：　　1. 周祥山，博士，教授，博士生導(dǎo)師，上海市科技啟明星，中組部“萬人計(jì)劃”第一批科技創(chuàng)新領(lǐng)軍人才。承擔(dān)國(guó)家自然科學(xué)基金和國(guó)家863計(jì)劃項(xiàng)目，主要從事微生物發(fā)酵和生物制藥的研究。　　2. 劉琴，博士，教授，博士生導(dǎo)師，上海市科技啟明星，教育部新世紀(jì)人才。承擔(dān)國(guó)家自然科學(xué)基金項(xiàng)目，參加國(guó)家863項(xiàng)目和國(guó)家支撐計(jì)劃項(xiàng)目，主要從事海洋生物技術(shù)的研究。　　3. 王啟要，博士，副教授，上海市科技啟明星。承擔(dān)國(guó)家自然科學(xué)基金項(xiàng)目，參加國(guó)家863項(xiàng)目、國(guó)家鲆鰈類農(nóng)業(yè)技術(shù)體系項(xiàng)目和國(guó)家公益性農(nóng)業(yè)行業(yè)專項(xiàng)，主要從事海洋生物技術(shù)的研究。　　4. 蔡孟浩，博士，副教授。承擔(dān)國(guó)家自然科學(xué)基金項(xiàng)目，參加國(guó)家863計(jì)劃研究項(xiàng)目，主要從事海洋微生物的研究。　　5. 周勉，博士，副教授。參加國(guó)家863計(jì)劃研究項(xiàng)目，主要從事微生物表達(dá)系統(tǒng)的研究。　　6. 馬悅，博士。承擔(dān)國(guó)家自然科學(xué)基金項(xiàng)目，參加國(guó)家公益性海洋行業(yè)專項(xiàng)和國(guó)家鲆鰈類農(nóng)業(yè)技術(shù)體系項(xiàng)目，主要從事海洋生物技術(shù)的研究。　　7. 肖婧凡，博士。承擔(dān)國(guó)家自然科學(xué)基金項(xiàng)目，參加國(guó)家863計(jì)劃研究項(xiàng)目，主要從事海洋生物技術(shù)的研究。　　8. 范衛(wèi)民，大學(xué)，高級(jí)工程師。參加國(guó)家863計(jì)劃研究項(xiàng)目，主要從事生物反應(yīng)器的研究和設(shè)計(jì)。　　9. 陸健，學(xué)士，高級(jí)工程師。參加國(guó)家863計(jì)劃研究項(xiàng)目，主要從事生物反應(yīng)器的研究和設(shè)計(jì)。　　10. 殷穎，博士后。主要從事海洋微生物的研究。　　11. 劉曉紅，師資博士后。主要從事海洋動(dòng)物疫苗的研究。　　12. 張靈芝，碩士。主要從事海洋病原微生物的研究。　　13. 林雅惠，學(xué)士。實(shí)驗(yàn)研究和實(shí)驗(yàn)室管理工作。

　　著作：

　　1. 王啟要，劉琴，張?jiān)d (2008) 群體感應(yīng)：細(xì)菌之間的語言. 葉勤主編：生物科學(xué)與工程. 北京：科學(xué)出版社. p43-67.

　　2. 張?jiān)d (2008) 動(dòng)物細(xì)胞培養(yǎng)工程. 葉勤主編：生物科學(xué)與工程. 北京：科學(xué)出版社. p160-171.

　　3. 張?jiān)d，易小萍，張立，孫祥明 (2007) 動(dòng)物細(xì)胞培養(yǎng)工程. 北京：化工出版社.

　　4. 張?jiān)d，許學(xué)書 (2001) 生物反應(yīng)器工程. 上海：華東理工大學(xué)出版社.

　　2015年發(fā)表和出版中論文：

　　1. Xiaohong Liu, Haizhen Wu*, Qin Liu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang (2015) Skin-injury zebrafish, Danio rerio, are more susceptible to Vibrio anguillarum infection. Journal of the World Aquaculture Society, accepted.

　　2. Shuai Shao, Qiliang Lai, Qin Liu, Haizhen Wu, Jingfan Xiao, Zongze Shao, Qiyao Wang*, Yuanxing Zhang (2015) Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: propose a novel species as Edwardsiella anguillarum sp. nov. Systematic and Applied Microbiology 38(1): 36-47.

　　3. Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu, Qiyao Wang*, Yuanxing Zhang (2015) An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Applied Microbiology and Biotechnology 99(4): 1765-1777.

　　4. Hua Zhang, Haizhen Wu*, Liang Gao, Ying Qiu, Jingfan Xiao, Yuanxing Zhang (2015) Identification, expression and immunological response to bacterial challenge following vaccination of BLT1 gene from turbot, Scophthalmus maximus. Gene 557(2): 229-235.

　　2014年以前發(fā)表的主要文章:

　　1. John S. Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang*, Zhiwei Song* (2014) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal 9(1): 100-109.

　　2. Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*, Yuanxing Zhang* (2014) Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. Journal of Microbiology and Biotechnology 24(2): 152-159.

　　3. Yingwei Liu, Xiangshan Zhou, Zhiwei Song*, Yuanxing Zhang* (2014) Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells. Biotechnology Letters 36(5): 907-911.

　　4. Haifeng Liu, Xiangshan Zhou*, Shousheng Tian, Xianghui Hao, Jinhua You, Yuanxing Zhang* (2014) Two-step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin-catalyzed cleavage and coupling. Biotechnology and Applied Biochemistry 61(4): 408-417.

　　5. Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2013) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Veterinary Microbiology 162(2-4): 652-662.

　　6. Haifeng Liu, Xiangshan Zhou*, Fusheng Xie, Jinhua You, Yuanxing Zhang* (2013) An efficient trypsin digestion strategy for improving desB30 productivity from recombinant human insulin precursor fusion protein. Process Biochemistry 48(5-6): 965-971.

　　7. Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2012) Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility, and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology 194(6): 439-452.

　　8. Ming Liu, Shuang Wang, Tingguang Sun, Jiguo Su, Yuanxing Zhang*, Junjie Yue*, Zhiwei Sun* (2012) Insight into the structure, dynamics and the unfolding property of amylosucrases: Implications of rational engineering on thermostability. PLoS One 7(7): e40441.

　　9. Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang* (2012) RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics 13: 319.

　　10. Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xunqian Sun, Yuanxing Zhang* (2012) An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology 14(6): 665-671.

　　11. Menghao Cai, Xueqian Sun, Xiangshan Zhou*, Yuanxing Zhang* (2012) Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus. Process Biochemistry 47(12): 2267-2274.

　　12. Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang, Yuanxing Zhang* (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology 102(3): 3584-3586.

　　13. Xiaodan Cao, Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu, Yuanxing Zhang* (2011) Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus. Microbial Pathogenesis 50(2):123-131.

　　14. Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Transient gene expression of a mouse homolog of Fc?-Fc? fusion protein for anti-allergic function assay. Process Biochemistry 46(4): 858-863.

　　15. Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang* (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology 30(3): 944-953.

　　16. Honghong He, Qiyao Wang, Lili Sheng, Qin Liu*, Yuanxing Zhang* (2011) Functional characterization of Vibrio alginolyticus twin-arginine translocation system: its roles in stress adaptation and virulence towards fish. Current Microbiology 62(4): 1193-1199.

　　17. Yanhua Pan, Wei Wei, Hongyan Xu, Qiyao Wang, Qin Liu, Haizhen Wu*, Yuanxing Zhang* (2011) Differential gene expression in Japanese flounder (Paralichthys olivaceus) induced by live attenuated Vibrio anguillarum. Aquaculture Research 42(7):1042-1049.

　　18. Huan Liu, Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi, Yuanxing Zhang* (2011) Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine. Applied Microbiology and Biotechnology 91(2):353-364.

　　19. Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Large-scale plasmid preparation for transient gene expression. Biotechnology Letters 33(8): 1559-1564.

　　20. Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang* (2011) Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology 13(5): 963-970.

　　21. Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, Yuanxing Zhang* (2011) Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology 53(4): 430-437.

　　22. Xiaonuo Teng, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2011) Modeling and application of controlled-fed perfusion culture of CHO cells in a bioreactor. Chemical and Biochemical Engineering Quarterly 25(3): 385-394.

　　23. Wei Wei, Haizhen Wu*, Hongyan Xu, Teng Chu, Changwen Wu, Kangmei Chang, Yuanxing Zhang* (2010) Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(1): 221-227.

　　24. Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou, Yuanxing Zhang* (2010) Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresource Technology 101(10):3609-3616.

　　25. Lingyun Zhou, Xiuhua Wang, Qin Liu*, Qiyao Wang, Yan Zhao, Yuanxing Zhang* (2010) A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila. Journal of Biotechnology 146(1-2): 25-30.

　　26. Haopeng Rui, Jennifer M. Ritchie, Roderick T. Bronson, John J. Mekalanos, Yuanxing Zhang, Matthew K. Waldor* (2010) Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins. Proceedings of the National Academy of Sciences of the United States of America 107(9):4359-4364.

　　27. Xiaodan Cao, Qiyao Wang, Qin Liu*, Huan Liu, Honghong He, Yuanxing Zhang* (2010) Vibrio alginolyticus MviN is a LuxO-regulated protein and affects cytotoxicity toward EPC cell. Journal of Microbiology and Biotechnology 20(2): 271-280.

　　28. Xiao Zhang, Wei Wei, Haizhen Wu*, Hongyan Xu, Kangmei Chang, Yuanxing Zhang* (2010) Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(5-6):735-742.

　　29. Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun, Yuanxing Zhang* (2010) Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresource Technology 101(15):6059-6068.

　　30. Cuihong Du, Xiaoping Yi, Yuanxing Zhang* (2010) expression and purification of soluble recombinant human endostatin in Escherichia coli. Biotechnology and Bioprocess Engineering 15(2):229-235.

　　31. Ping Zhang, Wenwen Zhang, Xiangshan Zhou*, Peng Bai, James M. Cregg, Yuanxing Zhang* (2010) Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. Applied and Environmental Microbiology 76(18): 6108-6118.

　　32. Xin Wang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Haizhen Wu, Yuanxing Zhang* (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish and Shellfish Immunology 29(6):1082-1091.

　　33. Suxia Li, Haizhen Wu, Jian Zhao, Ling Ou, Yuanxing Zhang* (2010) A biochemistry and molecular biology experiment and evaluation system for biotechnology specialty students. Biochemistry and Molecular Biology Education 38(4): 271-275.

　　34. Lili Xu, Qiyao Wang, Jingfan Xiao, Qin Liu*, Xin Wang, Tao Chen, Yuanxing Zhang* (2010) Characterization of Edwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Archives of Microbiology 192(12):1039-1047.

　　35. Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu*, Yuanxing Zhang* (2009) Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One 4(10): e7646.

　　36. Haopeng Rui, Qin Liu, Qiyao Wang, Yue Ma, Huan Liu, Cunbin Shi, Yuanxing Zhang* (2009) Role of alkaline serine protease Asp in Vibrio alginolyticus virulence and regulation of its expression by LuxO-LuxR regulatory system. Journal of Microbiology and Biotechnology 19(5): 431-438.

　　37. Wei Wei, Haizhen Wu, Hongyan Xu*, Tao Xu, Xiao Zhang, Kangmei Chang, Yuanxing Zhang* (2009) Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 27(2):285-295.

　　38. Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang* (2009) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish and Shellfish Immunology 27(3): 469-477.

　　39. Yuanxing Zhang (2009) Approaches to optimizing animal cell culture process: substrate metabolism regulation and protein expression improvement. Advances in Biochemical Engineering/Biotechnology 113: 177-215.

　　40. Qiyao Wang, Zhao Yang, Qin Liu*, Yuanxing Zhang* (2009) Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems. Aquaculture Research 40(13):1459-1467.

　　41. Jingfan Xiao, Qiyao Wang, Qin Liu, Xin Wang, Huan Liu, Yuanxing Zhang* (2009) Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquaculture Research 40(1):13-17.

　　42. Menghao Cai, Xiangshan Zhou*, Xueqian Sun, Kejing Tao, Yuanxing Zhang* (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. Journal of Industrial Microbiology & Biotechnology 36(3):381-389.

　　43. Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, Yuanxing Zhang* (2009) Characterization of Edwardsiella tarda rpoS: its roles in controlling serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Applied Microbiology and Biotechnology 83(1):151-160.

　　44. Wei Wei, Hongyan Xu*, Qiyao Wang, Xiao Zhang, Kangmei Chang, Changwen Wu, Yuanxing Zhang* (2009) Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum. Aquaculture 291(1-2):124-129.

　　45. Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao, Yuanxing Zhang* (2009) Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology 100(18):4244-4251.

　　46. Jing Ye, Yue Ma, Qin Liu, Dongling Zhao, Qiyao Wang, Yuanxing Zhang* (2008) Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases 31(3):161-169.

　　47. Chun Wei, Xiangshan Zhou, Yuanxing Zhang* (2008) Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme. Applied Microbiology and Biotechnology 78(2):257-264.

　　48. Zheyong Ma, Xiaoping Yi, Yuanxing Zhang* (2008) Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide. Process Biochemistry 43(6):690-695.

　　49. Weiyuan Zhang, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2008) Surface modification of non-woven poly (ethylene terephthalate) fabric scaffold for improving cell attachment in animal cell culture. Journal of Chemical Technology and Biotechnology 83(6):904-911.

　　50. Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Lingyu Guan, Yuanxing Zhang* (2008) Involvement of LuxS in the regulation of motility and flagellar biogenesis in Vibrio alginolyticus. Bioscience, Biotechnology, and Biochemistry 72(4): 1063-1071.

　　51. Lingyun Zhou, Qin Liu*, Qiyao Wang, Yue Ma, Yuzhou Xu, Zhao Yang, Yan Zhao, Yuanxing Zhang* (2008) Secretary delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design. Applied Microbiology and Biotechnology 79(6):1027-1034.

　　52. Zhao Yang, Qin Liu, Qiyao Wang, Yuanxing Zhang* (2008) Gene cloning and expression of major capsid protein of large yellow croaker iridovirus and its secretion based on ?-haemolysin transport system. Journal of the World Aquaculture Society 39(6):774-780.

　　53. Zhao Yang, Qin Liu*, Qiyao Wang, Yuanxing Zhang* (2008) Novel bacterial surface display systems based on outer membrane anchoring elements from the marine bacterium Vibrio anguillarum. Applied and Environmental Microbiology 74(14):4359-4365.

　　54. Ting Xie, Qin Liu*, Fusheng Xie, Haifeng Liu, Yuanxing Zhang* (2008) Secretory expression of insulin precursor in Pichia pastoris and simple procedure for producing recombinant human insulin. Preparative Biochemistry and Biotechnology 38(3):308-317.

　　55. Haopeng Rui, Qin Liu, Yue Ma, Qiyao Wang, Yuanxing Zhang* (2008) Roles of LuxR in extracellular alkaline serine protease A expression, extracellular polysaccharide production and mobility of Vibrio alginolyticus. FEMS Microbiology Letters 285(2):155-162.

　　56. Yuzhou Xu, Qin Liu*, Lingyun Zhou, Zhao Yang, Yuanxing Zhang* (2008) Surface display of GFP by Pseudomonas syringae truncated ice nucleation protein in attenuated Vibrio anguillarum strain. Marine Biotechnology 10(6):701-708.

　　57. Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Yuanxing Zhang* (2008) Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus. Archives of Microbiology 190(5): 585-594.

　　58. Qiyao Wang, Qin Liu, Xiaodan Cao, Minjun Yang, Yuanxing Zhang* (2008) Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus: their roles in iron utilization and virulence. Archives of Microbiology 190(5): 595-603.

　　59. Wenying Wang, Xiaoping Yi, Yuanxing Zhang* (2007) Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells. Biotechnology and Bioengineering 97(3):526-535.

　　60. Hongzhong Wu, Fan Yang, Shuya Cui, Yufeng Qin, Jianwen Liu*, Yuanxing Zhang* (2007) Hematopoietic effect of fractions from the enzyme-digested colla corii asini on mice with 5-fluorouracil induced anemia. The American Journal of Chinese Medicine 35(5): 853-866.

　　61. Lu Yang, Yue Ma, Yuanxing Zhang* (2007) Freeze-drying of live attenuated Vibrio anguillarum mutant for vaccine preparation. Biologicals 35(4):265-269.

　　62. Xiangming Sun, Yuanxing Zhang* (2007) A cell-detaching reactor for inoculation of anchorage-dependent CHO and Vero cells between stepwise-expanded bioreactors. Biotechnology Letters 29(5): 697-701.

　　63. Qin Liu, Pengbo Wang, Yue Ma, Yuanxing Zhang* (2007) Characterization of Vibrio alginolyticus fur gene and localization of essential amino acid sites in Fur by in vitro site-directed mutagenesis. Journal of Molecular Microbiology and Technology 13(1-3):15-21.

　　64. Liming Ouyang, Ling Ou, Yuanxing Zhang* (2007) An integrated teaching strategy for teaching biochemistry to biotechnology specialty students. Biochemistry and Molecular Biology Education 35 (4): 267-271.

　　65. Qiyao Wang, Qin Liu, Yue Ma, Haopeng Rui, Yuanxing Zhang* (2007) LuxO controls extracellular protease, hemolytic activities and siderophore production in fish pathogen Vibrio alginolyticus. Journal of Applied Microbiology 103(5):1525-1534.

　　66. Qiyao Wang, Qin Liu, Yue Ma, Yuanxing Zhang* (2007) Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and transportation of the siderophore of marine fish pathogen Vibrio alginolyticus. Archives of Microbiology 188(4):433-439.

　　67. Haiyan Huang, Yihua Yu, Xiaoping Yi, Yuanxing Zhang* (2007) Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy. Applied Microbiology and Biotechnology 77(2):427-436.

　　68. Fang Zhang, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2006) metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine. Cytotechnology 51(1):21-28.

　　69. Anfeng Xiao, Xiangshan Zhou, Li Zhou, Yuanxing Zhang* (2006) Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. Applied Microbiology and Biotechnology 72(4): 837-844.

　　70. Haiyan Huang, Xiaoping Yi, Yuanxing Zhang* (2006) Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds. Process Biochemistry 41(12):2386-2392.

　　71. Hechun Zhang, Jixun Zhan, Keman Su, Yuanxing Zhang* (2006) A kind of potential food additive produced by Streptomyces coelicolor: characteristics of blue pigment and identification of a novel compound, ?-actinorhodin. Food Chemistry 95(2):186-192.

　　72. Junhui Li, Zhengyu Huang, Xiangming Sun, Pengyuan Yang, Yuanxing Zhang* (2006) Understanding the enhanced effect of dimethyl sulfoxide on hepatitis B surface antigen expression in the culture of Chinese hamster ovary cells on the basis of proteome analysis. Enzyme and Microbiol Technology 38(3-4):372-380.

　　73. Junhui Li, Xiangming Sun, Yuanxing Zhang* (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochemistry 41(2):317-322.

　　74. Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2006) Gene cloning, expression and functional characterization of an acyl carrier protein AcpV from Vibrio anguillarum. Archives of Microbiology 185(2):159-163.

　　75. Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang* (2006) A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative. Biotechnology Letters 28(7):457-463.

　　76. Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2005) Gene cloning and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1. Achieves of Microbiology 183(1):37-44.

　　77. Subiao Lu, Xiangming Sun, Yuanxing Zhang* (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochemistry 40(5):1917-1921.

　　78. Juxing Chen, Xiangming Sun, Yuanxing Zhang* (2005) Growth and metabolism of marine fish Chinook salmon embryo cells: Response to lack of glucose and glutamine. Biotechnology Letters 27(6): 395-401.

　　79. Juxing Chen, Xiangming Sun, Li Zhang, Xiaoping Yi, Yuanxing Zhang* (2005) Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium. Aquaculture 249(1-4):35-45.

　　80. Mingfei Shao, Yue Ma, Qin Liu, Yuanxing Zhang* (2005) Secretory expression of recombinant proteins in an attenuated Vibrio anguillarum strain for potential use in vaccines. Journal of Fish Diseases 28(6):723-728.

　　81. Xiangming Sun, Yuanxing Zhang* (2004) Glutamine cannot support recombinant CHO cell growth and maintenance in the absence of glucose. Process Biochemistry 39(6):719-722.

　　82. Li Zhang, Hong Shen, Yuanxing Zhang* (2004) Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy. Journal of Chemical Technology and Biotechnology 79(2):171-181.

　　83. Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2004) Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression. Process Biochemistry 39(11):1817-1823.

　　84. Qin Liu, Yue Ma, Haizhen Wu, Mingfei Shao, Haifeng Liu, Yuanxing Zhang* (2004) Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1. Archives of Microbiology 181(4):287-293.

　　85. Weibin Zhou, Yuanxing Zhang* (2004) Purification and identification of recombinant hirudin and its degradation derivatives expressed in Pichia pastoris. Preparative Biochemistry & Biotechnology 34(3):239-252.

　　86. Jizhong Yang, Xiangshan Zhou, Yuanxing Zhang* (2004) Improvement of recombinant hirudin production by controlling NH4+ concentration in Pichia pastoris fermentation. Biotechnology Letter 26(12):1013-1017.

　　87. Yuanxing Zhang*, Lin Dai, Xiangming Sun (2003) Kinetic aspect of hepatitis B surface antigen production in recombinant Saccharomyces cerevisiae fermentation. Process Biochemistry 38(11):1593-1598.

　　88. Xiangming Sun, Yuanxing Zhang* (2003) Estimation of cell density in packed-bed bioreactor by lactate production rate. Biotechnology Letters 25(11):853-857.

　　89. Subiao Lu, Xiangming Sun, Chaoou Shi, Yuanxing Zhang* (2003) Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection. Journal of Chromatography A 1012(2):161-168.

　　90. Xiangshan Zhou, Jian Lu, Weimin Fan, Yuanxing Zhang* (2002) Development of a responsive methanol sensor and its application in Pichia pastoris fermentation. Biotechnology Letters 24(8): 643-646.

　　91. Xiangshan Zhou, Yuanxing Zhang* (2002) Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnology Letters 24(17):1449-1453.

　　92. Jingli Xie, Yuanxing Zhang*, Yonghong Wang, Yuanguang Li (2001) Mixotrophic cultivation of Platymonas subcordiformis. Journal of Applied Phycology 13(4):343-347.

　　93. Xiangming Sun, Yuanxing Zhang*, Wensong Tan, Yajing Zhou, Ping Hua (2000) Attachment kinetics of Vero cells onto CT3 microcarriers. Journal of Bioscience and Bioengineering 90(1):32-36.

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